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Selected Publications

 

Click on any of the publications below to read the abstract.

Graduate students are starred* and postdocs are denoted #

Brandt, A.M., Senkbeil, J.K., Lobos, A.E., Defillips, C., Lewis, D.B. and Harwood, V.J., 2025. Fecal indicator bacteria and sewage-associated marker genes are associated with nitrate and environmental parameters in two Florida freshwater systems. Journal of Applied Microbiology, p.lxaf030.

 

Lobos, A.E., Brandt, A.M., Gallard-Góngora, J.F., Korde, R., Brodrick, E. and Harwood, V.J., 2024. Persistence of sewage-associated genetic markers in advanced and conventional treated recycled water: implications for microbial source tracking in surface waters. Mbio, 15(7), pp.e00655-24.

Calarco, J., Pruden, A. and Harwood, V.J., 2024. Comparison of methods proposed for monitoring cefotaxime-resistant Escherichia coli in the water environment. Applied and Environmental Microbiology, 90(5), pp.e02128-23.

Keenum, I., Calarco, J., Majeed, H., Hager-Soto, E.E., Bott, C., Garner, E., Harwood, V.J. and Pruden, A., 2024. To what extent do water reuse treatments reduce antibiotic resistance indicators? A comparison of two full-scale systems. Water Research, 254, p.121425.

Liguori, K., Calarco, J., Maldonado Rivera, G., Kurowski, A., Keenum, I., Davis, B.C., Harwood, V.J. and Pruden, A., 2023. Comparison of Cefotaxime-Resistant Escherichia coli and sul 1 and int I1 by qPCR for Monitoring of Antibiotic Resistance of Wastewater, Surface Water, and Recycled Water. Antibiotics, 12(8), p.1252.

Milligan, E.G., Calarco, J., Davis, B.C., Keenum, I.M., Liguori, K., Pruden, A. and Harwood, V.J., 2023. A systematic review of culture-based methods for monitoring antibiotic-resistant Acinetobacter, Aeromonas, and Pseudomonas as environmentally relevant pathogens in wastewater and surface water. Current Environmental Health Reports, 10(2), pp.154-171.

Korajkic, A., McMinn, B.R. and Harwood, V.J., 2023. The effect of protozoa indigenous to lakewater and wastewater on decay of fecal indicator bacteria and coliphage. Pathogens, 12(3), p.378.

Davis, B.C., Keenum, I., Calarco, J., Liguori, K., Milligan, E., Pruden, A. and Harwood, V.J., 2022. Towards the standardization of Enterococcus culture methods for waterborne antibiotic resistance monitoring: A critical review of trends across studies. Water Research X, 17, p.100161.

Maiga, Y., Young, S., Orner, K.D., Mihelcic, J.R., Harwood, V.J. and Ouattara, A.S., 2022. Isolation and assessment of cyanide biodegradation potential of indigenous bacteria from contaminated soil. Journal of Environmental Protection, 13(10), pp.716-731.

Keenum, I., Liguori, K., Calarco, J., Davis, B.C., Milligan, E., Harwood, V.J. and Pruden, A., 2022. A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater. Critical Reviews in Environmental Science and Technology, 52(24), pp.4395-4419.

Liguori, K., Keenum, I., Davis, B.C., Calarco, J., Milligan, E., Harwood, V.J. and Pruden, A., 2022. Antimicrobial resistance monitoring of water environments: a framework for standardized methods and quality control. Environmental science & technology, 56(13), pp.9149-9160.

Gallard‐Gongora, J., Lobos, A., Conrad, J.W., Peraud, J. and Harwood, V.J., 2022. An assessment of three methods for extracting bacterial DNA from beach sand. Journal of Applied Microbiology, 132(4), pp.2990-3000.

Conrad, J.W. and Harwood, V.J., 2022. Sewage promotes Vibrio vulnificus growth and alters gene transcription in Vibrio vulnificus CMCP6. Microbiology spectrum, 10(1), pp.e01913-21.

Lobos, A., Harwood, V.J., Scott, K.M. and Cunningham, J.A., 2021. Tolerance of three fungal species to lithium and cobalt: Implications for bioleaching of spent rechargeable Li‐ion batteries. Journal of Applied Microbiology, 131(2), pp.743-755.

Conrad, J.W. and Harwood, V.J., 2021. Sewage Promotes Vibrio vulnificus Growth and Alters Gene Expression. bioRxiv, pp.2021-04.

Laureano-Rosario, A.E., Symonds, E.M., González-Fernández, A., Alvarado, D.M., Navarro, P.R., Badilla-Aguilar, A., Rueda-Roa, D., Otis, D.B., Harwood, V.J., Cairns, M.R. and Muller-Karger, F.E., 2021. The relationship between environmental parameters and microbial water quality at two Costa Rican beaches from 2002 to 2017. Marine Pollution Bulletin, 163, p.111957.

González-Fernández, A., Symonds, E.M., Gallard-Gongora, J.F., Mull, B., Lukasik, J.O., Navarro, P.R., Aguilar, A.B., Peraud, J., Brown, M.L., Alvarado, D.M. and Breitbart, M., 2021. Relationships among microbial indicators of fecal pollution, microbial source tracking markers, and pathogens in Costa Rican coastal waters. Water Research, 188, p.116507.

Nieuwkerk, D.M., Korajkic, A., Valdespino, E.L., Herrmann, M.P. and Harwood, V.J., 2020. Critical review of methods for isothermal amplification of nucleic acids for environmental analysis. Journal of microbiological methods, 179, p.106099.

Korajkic, A., McMinn, B.R., Staley, Z.R., Ahmed, W. and Harwood, V.J., 2020. Antibiotic-resistant Enterococcus species in marine habitats: A review. Current Opinion in Environmental Science & Health, 16, pp.92-100.

Young, S., Rohr, J.R. and Harwood, V.J., 2019. Vancomycin resistance plasmids affect persistence of Enterococcus faecium in water. Water research, 166, p.115069.

 

 

Korajkic A, Wanjugi P, Brooks L, Cao L, and Harwood VJ. 2019. Persistence and Decay of Fecal Microbiota in Aquatic Habitats. Microbiology and Molecular Biology Reviews 83.                   doi: 10.1128/MMBR.00005-19

Conrad JW, and Harwood VJ. 2019.  Comparison of DNA Methylation in Vibrio vulnificus Cells Grown in Human Serum with Those Grown in Seawater . Microbiology Resource Announcements 8. doi: 10.1128/MRA.00419-19 

Harwood VJ, and Murray N. 2019. Strategic discourse production and parent involvement: including parent knowledge and practices in the Lead My Learning campaign. International Journal of Inclusive Education 23:353–368. https://doi.org/10.1080/13603116.2019.1571119

Korajkic A, McMinn BR, Ashbolt NJ, Sivaganesan M, Harwood VJ, and Shanks OC. 2019. Extended persistence of general and cattle-associated fecal indicators in marine and freshwater environment. Science of the Total Environment 650:1292–1302.  https://doi.org/10.1016/j.scitotenv.2018.09.108

Senkbeil JK, Ahmed W, Conrad J, and Harwood VJ. 2019. Use of Escherichia coli genes associated with human sewage to track fecal contamination source in subtropical waters. Science of the Total Environment 686:1069–1075.  https://doi.org/10.1016/j.scitotenv.2019.05.201

Weiskerger CJ, Brandão J, Ahmed W, Aslan A, Avolio L, Badgley BD, Boehm AB, Edge TA, Fleisher JM, Heaney CD, Jordao L, Kinzelman JL, Klaus JS, Kleinheinz GT, Meriläinen P, Nshimyimana JP, Phanikumar MS, Piggot AM, Pitkänen T, Robinson C, Sadowsky MJ, Staley C, Staley ZR, Symonds EM, Vogel LJ, Yamahara KM, Whitman RL, Solo-Gabriele HM, and Harwood VJ. 2019. Impacts of a changing earth on microbial dynamics and human health risks in the continuum between beach water and sand. Water Research 162:456-470. https://doi.org/10.1016/j.watres.2019.07.006

Young S, Rohr JR, and Harwood VJ. 2019. Vancomycin resistance plasmids affect persistence of Enterococcus faecium in water. Water Research 166:115069.  https://doi.org/10.1016/j.watres.2019.115069

Zhang Q, Gallard J, Wu B, Harwood VJ, Sadowsky MJ, Hamilton KA, and Ahmed W. 2019. Synergy between quantitative microbial source tracking (qMST) and quantitative microbial risk assessment (QMRA): A review and prospectus. Environment International 130:104703.  https://doi.org/10.1016/j.envint.2019.03.051

Korajkic A, McMinn BR, and Harwood VJ. 2018. Relationships between microbial indicators and pathogens in recreational water settings. International Journal of Environmental Research and Public Health 15:2842. https://doi.org/10.3390/ijerph15122842

K.H. Nguyen*, C. Senay, S. Young*, B. Nayak, A. Lobos*, J. Conrad*, and V.J. Harwood. 2018. Determination of Wild Animal Sources of Fecal Indicator Bacteria by Microbial Source Tracking (MST) Influences Regulatory Decisions. Water Research 144: 424-434. doi: 10.1016/j.watres.2018.07.034.
 
Symonds EM, Nguyen KH, Harwood VJ, and Breitbart M. 2018. Water Research 144:1-12. https://doi.org/10.1016/j.watres.2018.06.066
Warish A, Zhang Q, Lobos A, Senkbeil J, Sadowsky MJ, Harwood VJ, Saeidi N, Marinoni O, Ishii S. 2018. Precipitation influences pathogenic bacteria and antibiotic resistance genes abundance in storm drain outfalls in coastal sub-tropical waters. Environment International 116:308-318. https://doi.org/10.1016/j.envint.2018.04.005
Ahmed W, Hamilton KA, Lobos A, Hughes B, Staley C, Sadowsky MJ, Harwood VJ. 2018. Quantitative microbial risk assessment of microbial source tracking markers in recreational water contaminated with fresh untreated and secondary treated sewage. Environment International 117:243-249. https://doi.org/10.1016/j.envint.2018.05.012
Ahmed W, Lobos A, Senkbeil J, Peraud J, Gallard J, Harwood VJ. 2018. Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida. Water Research 131:142-150. https://doi.org/10.1016/j.watres.2017.12.011
Staley C, Kaiser T, Lobos A, Ahmed W, Harwood VJ, Brown CM, Sadowsky MJ. 2018. Application of SourceTracker for accurate identification of fecal pollution in recreational freshwater: a double-blinded study. Environmental Science & Technology 52 (7), 4207-4217. doi: 10.1021/acs.est.7b05401
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