Selected Publications
Click on any of the publications below to read the abstract.
Graduate students are starred* and postdocs are denoted #
Brandt, A.M., Senkbeil, J.K., Lobos, A.E., Defillips, C., Lewis, D.B. and Harwood, V.J., 2025. Fecal indicator bacteria and sewage-associated marker genes are associated with nitrate and environmental parameters in two Florida freshwater systems. Journal of Applied Microbiology, p.lxaf030.
Lobos, A.E., Brandt, A.M., Gallard-Góngora, J.F., Korde, R., Brodrick, E. and Harwood, V.J., 2024. Persistence of sewage-associated genetic markers in advanced and conventional treated recycled water: implications for microbial source tracking in surface waters. Mbio, 15(7), pp.e00655-24.
Calarco, J., Pruden, A. and Harwood, V.J., 2024. Comparison of methods proposed for monitoring cefotaxime-resistant Escherichia coli in the water environment. Applied and Environmental Microbiology, 90(5), pp.e02128-23.
Keenum, I., Calarco, J., Majeed, H., Hager-Soto, E.E., Bott, C., Garner, E., Harwood, V.J. and Pruden, A., 2024. To what extent do water reuse treatments reduce antibiotic resistance indicators? A comparison of two full-scale systems. Water Research, 254, p.121425.
Liguori, K., Calarco, J., Maldonado Rivera, G., Kurowski, A., Keenum, I., Davis, B.C., Harwood, V.J. and Pruden, A., 2023. Comparison of Cefotaxime-Resistant Escherichia coli and sul 1 and int I1 by qPCR for Monitoring of Antibiotic Resistance of Wastewater, Surface Water, and Recycled Water. Antibiotics, 12(8), p.1252.
Milligan, E.G., Calarco, J., Davis, B.C., Keenum, I.M., Liguori, K., Pruden, A. and Harwood, V.J., 2023. A systematic review of culture-based methods for monitoring antibiotic-resistant Acinetobacter, Aeromonas, and Pseudomonas as environmentally relevant pathogens in wastewater and surface water. Current Environmental Health Reports, 10(2), pp.154-171.
Korajkic, A., McMinn, B.R. and Harwood, V.J., 2023. The effect of protozoa indigenous to lakewater and wastewater on decay of fecal indicator bacteria and coliphage. Pathogens, 12(3), p.378.
Davis, B.C., Keenum, I., Calarco, J., Liguori, K., Milligan, E., Pruden, A. and Harwood, V.J., 2022. Towards the standardization of Enterococcus culture methods for waterborne antibiotic resistance monitoring: A critical review of trends across studies. Water Research X, 17, p.100161.
Maiga, Y., Young, S., Orner, K.D., Mihelcic, J.R., Harwood, V.J. and Ouattara, A.S., 2022. Isolation and assessment of cyanide biodegradation potential of indigenous bacteria from contaminated soil. Journal of Environmental Protection, 13(10), pp.716-731.
Keenum, I., Liguori, K., Calarco, J., Davis, B.C., Milligan, E., Harwood, V.J. and Pruden, A., 2022. A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater. Critical Reviews in Environmental Science and Technology, 52(24), pp.4395-4419.
Liguori, K., Keenum, I., Davis, B.C., Calarco, J., Milligan, E., Harwood, V.J. and Pruden, A., 2022. Antimicrobial resistance monitoring of water environments: a framework for standardized methods and quality control. Environmental science & technology, 56(13), pp.9149-9160.
Gallard‐Gongora, J., Lobos, A., Conrad, J.W., Peraud, J. and Harwood, V.J., 2022. An assessment of three methods for extracting bacterial DNA from beach sand. Journal of Applied Microbiology, 132(4), pp.2990-3000.
Conrad, J.W. and Harwood, V.J., 2022. Sewage promotes Vibrio vulnificus growth and alters gene transcription in Vibrio vulnificus CMCP6. Microbiology spectrum, 10(1), pp.e01913-21.
Lobos, A., Harwood, V.J., Scott, K.M. and Cunningham, J.A., 2021. Tolerance of three fungal species to lithium and cobalt: Implications for bioleaching of spent rechargeable Li‐ion batteries. Journal of Applied Microbiology, 131(2), pp.743-755.
Conrad, J.W. and Harwood, V.J., 2021. Sewage Promotes Vibrio vulnificus Growth and Alters Gene Expression. bioRxiv, pp.2021-04.
Laureano-Rosario, A.E., Symonds, E.M., González-Fernández, A., Alvarado, D.M., Navarro, P.R., Badilla-Aguilar, A., Rueda-Roa, D., Otis, D.B., Harwood, V.J., Cairns, M.R. and Muller-Karger, F.E., 2021. The relationship between environmental parameters and microbial water quality at two Costa Rican beaches from 2002 to 2017. Marine Pollution Bulletin, 163, p.111957.
González-Fernández, A., Symonds, E.M., Gallard-Gongora, J.F., Mull, B., Lukasik, J.O., Navarro, P.R., Aguilar, A.B., Peraud, J., Brown, M.L., Alvarado, D.M. and Breitbart, M., 2021. Relationships among microbial indicators of fecal pollution, microbial source tracking markers, and pathogens in Costa Rican coastal waters. Water Research, 188, p.116507.
Nieuwkerk, D.M., Korajkic, A., Valdespino, E.L., Herrmann, M.P. and Harwood, V.J., 2020. Critical review of methods for isothermal amplification of nucleic acids for environmental analysis. Journal of microbiological methods, 179, p.106099.
Korajkic, A., McMinn, B.R., Staley, Z.R., Ahmed, W. and Harwood, V.J., 2020. Antibiotic-resistant Enterococcus species in marine habitats: A review. Current Opinion in Environmental Science & Health, 16, pp.92-100.
Young, S., Rohr, J.R. and Harwood, V.J., 2019. Vancomycin resistance plasmids affect persistence of Enterococcus faecium in water. Water research, 166, p.115069.
Conrad JW, and Harwood VJ. 2019. Comparison of DNA Methylation in Vibrio vulnificus Cells Grown in Human Serum with Those Grown in Seawater . Microbiology Resource Announcements 8. doi: 10.1128/MRA.00419-19